Digital Histology Interest Group or DHIG

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  • 1.  Alternatives to Aperio for digital histology

    Posted 05-05-2021 09:05

    Dear colleagues,

    I am writing on behalf of our team of histology instructors to learn what platforms different schools are using for their digital histology. We have been using Aperio with Spectrum Education for some time, but have learned that Leica/Aperio is moving away from education. As a result, we are losing the support that we have relied on up until now. At this juncture, we are wondering what other platforms might be available as an alternative - one that would support our collection of slides and would function on both Windows and Apple based operating systems. I would appreciate any suggestions that the group might have.

    Thanks so much,

    Bryon



    ------------------------------
    Bryon Grove, Ph.D.
    Associate Professor,
    Biomedical Sciences,
    UND School of Medicine/Hlth Sci
    Grand Forks, ND
    701-777-2579/bryon.grove@und.edu
    ------------------------------


  • 2.  RE: Alternatives to Aperio for digital histology

    Posted 05-05-2021 09:08
    Hello,

    I am also interest in this questions as I am moving to a School of Dental medicine and one of my goals is to establish virtual microscopy.

    I am looking forward to your answers.

    Best,

    Alicia

    ------------------------------
    Alicia De Maria
    Instructor
    Washington University
    Saint Louis MO
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  • 3.  RE: Alternatives to Aperio for digital histology

    Posted 05-06-2021 09:00
    Hello Alicia and Byron and everybody else,
    Luckily there are a number of options for virtual microscopy software, may be too many. Most likely your choice will boil down to three variables: a) Money available; b) your time and technical knowledge; and c) the availability of a supportive IT group at your institution. If money is not an issue, I can recommend MBF Bioscience. They support the VMD and their Biolucida viewer is free and will work with svs files. You can contact Dr. Nate O'Connor at
    Please mention that you are contacting him because of a recommendation from the VMD.
    I recently had to replace the old Flash-dependent old Aperio viewer software for the Michigan Histology Website (Histology at the University of Michigan ). Based on Haviva's recommendation we settled on a subscription with Zoomify (Zoomify - Zoomable web images! ). Not too expensive, but their viewer will not work with svs files and my IT people had to convert all of our svs files. Unless you have thousands of files, that is not too bad as Zoomify has a file converter. There are more viewer software option, some may even be available for free, but most will require a file conversion. 
    I hope this will give you some ideas and other DHIG colleagues may have additional options they can recommend.
    All the best
    Michael



    ------------------------------
    Michael Hortsch
    University of Michigan
    Ann Arbor MI
    734-647 2720
    hortsch@umich.edu
    ------------------------------



  • 4.  RE: Alternatives to Aperio for digital histology

    Posted 05-06-2021 08:29
    Edited by Robert Ogilvie 05-06-2021 08:30
    Hi Byron and Alicia

    I have been using Biolucida software sold, distributed and supported by MBFbioscience.com.  
    MBF Bioscience | Stereology and Neuron Morphology Quantitative Analysis

    They have multiple products for image hosting, image analysis, and software for education to host whole (virtual) slides.  The product that you would be interested in is Biolucida.  Here is a link to learn about that.  You find it under the drop down menu for their products.

    https://www.mbfbioscience.com/biolucida   You will see that they have a Biolucida product for Education, Research, and publication.

    I have been teaching and managing an online course in histology for the University of South Carolina year around for 10 years using virtual slides hosted by a server on campus with Biolucida server software installed.  

    MBFbioscience hosts using their Biolucida software and server the American Association of Anatomy Virtual Microscopy Database that you can access here:  VMD Websitehttp://www.virtualmicroscopydatabase.org/ 

    Perhaps you are already a user but, if not, you can join and peruse the extensive collection.  

    Feel free to contact me if you have questions.  You can phone MBFbioscience at this number to inquire and request a demonstration:

    (802) 288-9290   You can also contact Dr. Michael Hortsch, University of Michigan.  He is very much involved with the AAA Virtual Microscopy Database and is familiar with Biolucida.  You can reach him by email at hortsch@med.uich.edu or by phone at (734) 647-2720.

    My experience with Whole (Virtual) Slides scanning, hosting, and use for medical education goes back to 2000.  In 2002 four years before I retired from my fulltime tenured faculty position in the Medical University of South Carolina, I organized what I believe to be the first ever Symposium on Virtual Slides in Teaching, Diagnosis, and Research with over 100 attendees United States, Canada, Japan, and Ireland.  I had all of the early commercial technology companies in the field of Virtual Microscopy, including Aperio, attending and giving talks.

    At this point I continue to teach the histology course year around for the University of South Carolina and also each summer a similar online histology course for Graduate Students at the Medical University of South Carolina.

    If you would like a demonstration of how I use Biolucida software and the client side software, Biolucida Viewer, in my courses, we could arrange for you to visit my desktop while we talk by phone.  

    With kind regards,

    Bob Ogilvie

    ------------------------------
    Robert W. Ogilvie, PhD
    Visiting Professor, University of South Carolina
    Professor Emeritus, Medical University of South Carolina
    Columbia & Charleston, South Carolina
    Cell Phone: (843) 693-1065
    Email: rogilvie31@yahoo.com
    ------------------------------



  • 5.  RE: Alternatives to Aperio for digital histology

    Posted 05-06-2021 09:40
    Bryon:

    We use a home-built Virtual Microscope system which is publicly available.  There are two entry points into the system:  http://viewer.pnwu.edu opens the slide viewer (i.e., the virtual microscope), and http://slidebox.pnwu.edu opens a searchable listing of the available slides.   The motivation for building this system was to allow us to use high-resolution multifocal-plane virtual slides for teaching (described in a poster at EB2020: http://viewer.pnwu.edu/docs/poster_EB2020.pdf), but it is designed to also handle the 'usual' single-focal-plane slides.  The user manual for the viewer is at http://viewer.pnwu.edu/docs/Viewer_User_Manual.pdf (it also is accessible from the About menu on the Viewer).  The software that runs Viewer & SlideBox consists of HTML, javascript, and php files which are available for free under a GNU GPL license, and have been posted to GitHub (i.e., no proprietary software); it also uses a mySQL database (the contents of which would be specific to your database of virtual slides).  I apologize for the blatant PNWU advertising embedded in the system, but it was a way of convincing the University that the viewer should be on the public side of our website.

    SlideBox is intended principally for instructors when they are building their labs.  Students access the Viewer directly through links embedded in the lab handout (which is a PDF in our case), and there is no need for the students to know anything about the underlying database.  The link in the lab handout looks something like:  "Next, look at slide UM130-75 at low-to-intermediate magnification and identify the seminal vesicle, the prostate gland, and the ampulla of the ductus deferens.", where the link attached to "UM130-75" is http://viewer.pnwu.edu/?slide=3005 (when the link has this form, the students don't even see the "choose a new slide" option).  The system has the ability to open the slide at a particular x,y location, focal plane, and zoom-level, so the lab handout could also have the form:  "Within the muscle spindle (on slide UM145-59), note the nuclear bag fiber (cell) and nuclear chain fibers (cells)" where the link http:viewer.pnwu.edu?slide=3008 & x=136000 & y=68000 & f=10 & z=8 specifies not only the slide, but also a specific location (and zoom-level) within the slide.

    Prior to building our own virtual microscope system, and in anticipation of developing that system, we had previously used the NYU Google-maps-based system.  I'm currently in the process of 'moving' our set of teaching slides from the old system into the new system, so SlideBox currently only displays a rather lopsided set of teaching slides (I've almost finished importing all of our Male Reproductive System slides, but most of the other systems are pretty sparse right now), but it should give you an idea of where the system is headed ... in SlideBox, I'll add drop-down submenus under in "Limit Slides by ... Organ System" that will allow the user to select slides of specific organs (i.e., under "Male Reproductive System, I'll add a submenu that will allow the user to select 'all male reproductive system slides', 'testis', 'ductuli efferentes', 'epididymis', 'ductus deferens', 'seminal vesicle', 'prostate gland', 'bulbourethral gland', and 'penis').

    Currently the implementation of the Viewer and SlideBox on the PNWU website are available for anyone to use.  I will be retiring from PNWU at the end of June.  The decision to retire was rather precipitate, so I have no idea regarding the fate of the PNWU's implementation of the Virtual Microscope or where/how I will continue development of this system (although I really would like to continue to develop this system, including adding many more slides to the database).  The underlying software is available on GitHub (and is free under the GPL license), so the system is available to our community regardless of what happens to PNWU's implementation.  Perhaps more significantly, the system is NOT dependent on proprietary software so we should never have to worry about a vendor leaving us in a lurch.

    Hope this is helpful.

          - - Jim

    ------------------------------
    James Rhodes
    Pacific Northwest University of Health Sciences
    Yakima WA
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  • 6.  RE: Alternatives to Aperio for digital histology

    Posted 05-07-2021 02:11
    Hello Everyone.
    I have developed a series of extensive videos describing the histology of human cells, tissue and organs - https://www.meyershistology.com
    and an online interactive histology atlas - https://www.histology-online.com which is accessed by students/universities worldwide.
    The online interactive atlas has embedded in it a series of histological slides that the student/user can examine using virtual microscopy. The sections were originally scanned and converted to "ZIF" files using http://www.zoomify.com for zoomable web images, and particularly David Urbanic was most helpful.and I use "The ObjectiveView digital pathology viewing software" for both Microsoft Windows and Apple Mac OS X to view sections. 
    I am currently reloading a lot more scanned histological sections into the atlas.
    I am also redoing the video series to use virtual microscopy rather than still images.
    I am loading a whole lot more quizzes questions for each topic.
    I use both these resources to teach histology completely online.

    ------------------------------
    Geoffrey Meyer
    Professor
    University of Western Australia
    Perth WA
    ------------------------------



  • 7.  RE: Alternatives to Aperio for digital histology

    Posted 05-10-2021 11:53
    Hi Bryon & Alicia,
    One virtual microscope that you could look at is the virtual microscope that I built at Tulane in 2013 -- https://virtualmicroscopy.tulane.edu (with help from Jim Rhodes at PNWU in converting slides). Access is completely free. The VM is built on GoogleMaps API and works on desktop and mobile devices.

    ------------------------------
    Raj Ettarh
    California University of Science and Medicine
    Colton CA
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  • 8.  RE: Alternatives to Aperio for digital histology

    Posted 05-10-2021 21:53
    Hi all,

    At Duke, we have switched over to using DigitalScope (https://digitalscope.org).  The viewer is HTML5-based and works on just every browser I have tried (Mac, PC, Android, and iOS devices).  They host the slides for us for a yearly fee that is about the same as what we were charged by our IT department and the software agreement from Aperio back when we had our own servers running ImageServer.

    The folks at DigitalScope set us up with a dashboard where we can upload and manage our slide collection without much trouble. They can also work with a variety of slide formats such as .svs (the Aperio format), .jp2, bigtiff, Zeiss, Olympus, Perkin Elmer, and Hamamatsu.  So far, it's been a fairly easy process and it's nice not having to bother with setting up our own servers. 

    I've been working on updating both our histology (https://histology.oit.duke.edu/) and pathology (https://pathology.oit.duke.edu/) sites with links to DigitalScope versions of our slides if anyone would like to check them out (our sites are open to the world, so anyone is welcome to use them from anywhere).  Alternatively, here's a direct link to one of our slides: https://www.digitalscope.org/LinkHandler.axd?LinkId=76173118-4572-43be-a1fa-fcdcc506fc70&ann=11100001101 (the left side of the viewer has various tools that you can turn on or off, including the "smooth panning" feature which I don't particuarly care for but some folks seem to like....)

    If anyone has any questions, I'd be happy to share our experiences going through the process of going to a non-Flash format.

    Best,
    Matt Velkey

    ------------------------------
    Matt Velkey
    Duke University
    Durham NC
    matt.velkey@duke.edu
    (734) 646-8320

    ------------------------------
    John Velkey
    Duke University
    Durham NC
    (734) 646-8320
    ------------------------------



  • 9.  RE: Alternatives to Aperio for digital histology

    Posted 05-23-2021 08:41
    Hello,
     I am at Nova Southeastern University in Florida. We are using Biolucida for our virtual histology courses which together includes almost 800 MD, DO, Dental and Master's students on two campuses. It has worked well for us. It is especially easy to use the Virtual Histology database since the same company handles both.

    Lori Dribin
    Professor
    Nova Southeastern University
    Kiran C. Patel College of Osteopathic Medicine

    lorib@nova.edu

    ------------------------------
    Lori Dribin
    Professor of Anatomy
    Nova Southeastern University
    College of Medical Sciences]
    Fort Lauderdale, FL
    Work>954-262-1341
    Cell 305-331-2857

    lorib@nova.edu(954) 262- 1341
    ------------------------------